diff --git a/sources b/sources
index e17d906..f61ddfc 100644
--- a/sources
+++ b/sources
@@ -1 +1 @@
-SHA512 (ugene-free-53.1.tar.gz) = 5d2a1d9fac81a14fd7916c33223704aaaedfc29bd39086561202766f87cebbc7739775eccabe6a789a2381e14384bd7f8f4f7a9dfde334811190fe47dbbcc455
+SHA512 (ugene-free-20260906T1440.tar.gz) = fc0890fc79acb337959450119b81b5178ba0f3ff3fb7502e1450d12d0e4f18a8a0586b5b6256d9d97c42f21251bbc54c97d215a23fb68d1e3fd33368fe1da37f
diff --git a/ugene-53.1.x-qmin-arg-signness.patch b/ugene-53.1.x-qmin-arg-signness.patch
new file mode 100644
index 0000000..5e3b0f1
--- /dev/null
+++ b/ugene-53.1.x-qmin-arg-signness.patch
@@ -0,0 +1,17 @@
+diff --git a/src/plugins/pcr/src/InSilicoPcrTask.cpp b/src/plugins/pcr/src/InSilicoPcrTask.cpp
+index c2e106cd9..a5febc83e 100644
+--- a/src/plugins/pcr/src/InSilicoPcrTask.cpp
++++ b/src/plugins/pcr/src/InSilicoPcrTask.cpp
+@@ -66,10 +66,10 @@ namespace {
+ int getMaxError(const InSilicoPcrTaskSettings* settings, U2Strand::Direction direction) {
+     int res = 0;
+     if (direction == U2Strand::Direct) {
+-        res = qMin((qsizetype)settings->forwardMismatches, settings->forwardPrimer.length() - settings->perfectMatch);
++        res = qMin((qsizetype)settings->forwardMismatches, settings->forwardPrimer.length() - (qsizetype)settings->perfectMatch);
+         res = qMin(res, settings->forwardPrimer.length() - 1);
+     } else {
+-        res = qMin((qsizetype)settings->reverseMismatches, settings->reversePrimer.length() - settings->perfectMatch);
++        res = qMin((qsizetype)settings->reverseMismatches, settings->reversePrimer.length() - (qsizetype)settings->perfectMatch);
+         res = qMin(res, settings->reversePrimer.length() - 1);
+     }
+     return qMax(0, res);
diff --git a/ugene.spec b/ugene.spec
index 7332607..e187721 100644
--- a/ugene.spec
+++ b/ugene.spec
@@ -2,10 +2,10 @@
 
 %if 0%{?use_release_branch} < 1
 # master
-%global	gitdate		20260228
-%global	gitcommit		77338a76808d0e1b36461a94d16f0a45c83b64e9
+%global	gitdate		20260905
+%global	gitcommit		0a5caecdcd44ea72e322524032ee8bc8b94f901a
 # New git commit with non-free part removed using "git filter-branch"
-%global	gitcommit_free		6812b00935495786e28ebe901bfb8304dbee457c
+%global	gitcommit_free		84beedcc4e5b93b227a96cc8303a4d56ac82ca64
 %else
 # currently 41.0 branch
 %global	gitdate		20250313
@@ -18,11 +18,11 @@
 %global	shortcommit	%(c=%{gitcommit}; echo ${c:0:7})
 %global	git_version	%{gitdate}git%{shortcommit}
 
-%global	tarballdate	20260301
-%global	tarballtime	1637
+%global	tarballdate	20260906
+%global	tarballtime	1440
 
-%global	use_release	1
-%global	use_gitbare	0
+%global	use_release	0
+%global	use_gitbare	1
 
 %if	0%{?use_gitbare} < 1
 # force
@@ -50,7 +50,7 @@ Name:		ugene
 Summary:	Integrated bioinformatics toolkit
 
 Version:	%{fedoraver}
-Release:	2%{?dist}
+Release:	1%{?dist}
 
 #The entire source code is GPLv2+ except:
 #file src/libs_3rdparty/qtbindings_core/src/qtscriptconcurrent.h which is GPLv2
@@ -73,6 +73,7 @@ Source2:	create-%{name}-git-bare-tarball.sh
 Source10:	ugene.wrapper
 Patch1:	ugene-49.1-narrowing-for-unsigned-char.patch
 Patch3:	ugene-52.1.x-QObject-connect-overload.patch
+Patch4:	ugene-53.1.x-qmin-arg-signness.patch
 # Currently distro-specific
 Patch102:	ugene-44.x-libs_3rdparty-breakpad-sys_mmap_use_system_mmap.patch
 Patch103:	ugene-40.1-libs_3rdparty-breakpad-unwind-nonsupported-arch.patch
@@ -91,20 +92,21 @@ BuildRequires:	desktop-file-utils
 BuildRequires:	%{_bindir}/git
 %endif
 
-BuildRequires:	cmake(Qt5Core)
-BuildRequires:	cmake(Qt5Gui)
-BuildRequires:	cmake(Qt5LinguistTools)
-BuildRequires:	cmake(Qt5Network)
-BuildRequires:	cmake(Qt5NetworkAuth)
-BuildRequires:	cmake(Qt5PrintSupport)
-BuildRequires:	cmake(Qt5Script)
-BuildRequires:	cmake(Qt5ScriptTools)
-BuildRequires:	cmake(Qt5Sql)
-BuildRequires:	cmake(Qt5Svg)
-BuildRequires:	cmake(Qt5Test)
-BuildRequires:	cmake(Qt5WebSockets)
-BuildRequires:	cmake(Qt5Widgets)
-BuildRequires:	cmake(Qt5Xml)
+BuildRequires:	cmake(Qt6Core)
+BuildRequires:	cmake(Qt6Core5Compat)
+BuildRequires:	cmake(Qt6CorePrivate)
+BuildRequires:	cmake(Qt6Gui)
+BuildRequires:	cmake(Qt6LinguistTools)
+BuildRequires:	cmake(Qt6Network)
+BuildRequires:	cmake(Qt6NetworkAuth)
+BuildRequires:	cmake(Qt6OpenGL)
+BuildRequires:	cmake(Qt6OpenGLWidgets)
+BuildRequires:	cmake(Qt6PrintSupport)
+BuildRequires:	cmake(Qt6Svg)
+BuildRequires:	cmake(Qt6Test)
+BuildRequires:	cmake(Qt6WebSockets)
+BuildRequires:	cmake(Qt6Widgets)
+BuildRequires:	cmake(Qt6Xml)
 
 BuildRequires:	pkgconfig(gl)
 BuildRequires:	pkgconfig(glu)
@@ -140,6 +142,8 @@ git config user.email "%{name}-maintainers@fedoraproject.org"
 	%GIT commit -m "Fix narrowing on arch where default char is unsigned" -a
 %patch -P3 -p1 -b .include
 	%GIT commit -m "RegionSelectorController: specify overloaded function" -a
+%patch -P4 -p1 -b .signness
+	%GIT commit -m "Fix signness for qMin argument" -a
 %patch -P102 -p1 -b .sys_mmap -Z
 	%GIT commit -m "libs_3rdparty/breakpad: use C function instead of directly using syscall assemble code" -a
 %patch -P103 -p1 -b .unwind -Z